Bioxys

Rabbit Polyclonal IgG Anti-VEGF-A, pan-specific

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Catalog number:VEGA01
Name:Rabbit Polyclonal IgG Anti-VEGF-A, pan-specific
Size:100 µg
Price:262.00
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Availability:Please contact us to check the availability
Immunogen:Highly purified human N-terminal VEGF-A (20 aa peptide)
Specificity:VEGF-A isoforms in human, mouse and rat,
Species reactivity:Human, Mouse, Rat
Product presentation:Protein A purified IgG lyophilised from PBS pH 7.4 without preservative
Product category:Primary Antibody
Antibody's raised in:Rabbit
Antibody's isotype:IgG
Antibody's clone:polyclonal
Product is supplied as:lyophilisate
Recommended concentration for use:5-10 µg/ml (WB), 0.5-2 µg/ml (dir. ELISA)
Protein concentration:100 µg
Positive control:Metastasing tumours of different origin
Tested applications:ELISA, WB (red. cond.), IHC not tested
Recommended secondary reagent:As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
Storage method:Store at -20°C
UniProt number:P15692 (VEGFA_HUMAN)
Litterature:1. Gu W, Brannstrom T, Jiang W, Bergh A, Wester P. Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion. Acta Neuropathol (Berl). 2001 Sep;102(3):216-26. 2. Tille JC, Wang X, Lipson KE, McMahon G, Ferrara N, Zhu Z, Hicklin DJ, Sleeman JP, Eriksson U, Alitalo K, Pepper MS. Vascular endothelial growth factor (VEGF) receptor-2 signaling mediates VEGF-C(deltaNdeltaC)- and VEGF-A-induced angiogenesis in vitro. Exp Cell Res. 2003 May 1;285(2):286-98. 2. Tille JC, Wang X, Lipson KE, McMahon G, Ferrara N, Zhu Z, Hicklin DJ, Sleeman JP, Eriksson U, Alitalo K, Pepper MS. Vascular endothelial growth factor (VEGF) receptor-2 signaling mediates VEGF-C(deltaNdeltaC)- and VEGF-A-induced angiogenesis in vitro. Exp Cell Res. 2003 May 1;285(2):286-98.
Tips:Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype:IgG
Group:Polyclonals and antibodies
Latin name:Oryctolagus cuniculus

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