Rabbit Polyclonal IgG Anti-VEGF-C, rat (human)

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Catalog number:VEGC02
Name:Rabbit Polyclonal IgG Anti-VEGF-C, rat (human)
Size:100 µg
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Availability:Please contact us to check the availability
Immunogen:Recombinant rat delta-N-delta-C-VEGF-C (native protein)
Specificity:Rat VEGF-C, no reactivity with VEGF-A and -D
Species reactivity:Rat, cross-reaction with mouse and human
Product presentation:Protein A purified IgG lyophilised from PBS pH 7.4 without preservative
Product category:Primary Antibody
Antibody's raised in:Rabbit
Antibody's isotype:IgG
Antibody's clone:polyclonal
Product is supplied as:lyophilisate
Recommended concentration for use:ELISA>2.5 µg/ml, Immunoprecipitation 1-10 µg/ml lysate or reaction volume, IHC not tested
Protein concentration:100 µg
Positive control:Metastasing tumours of different origin
Tested applications:ELISA, IP, IHC not tested
Recommended secondary reagent:As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
Storage method:Store at -20°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Fitz LJ, Morris JC, Towler P, Long A, Burgess P, Greco R, Wang J, Gassaway R, Nickbarg E, Kovacic S, Ciarletta A, Giannotti J, Finnerty H, Zollner R, Beier DR, Leak LV, Turner KJ, Wood CR. Characterization of murine Flt4 ligand/VEGF-C. Oncogene. 1997 Jul 31;15(5):613-8. 2. Gu W, Brannstrom T, Jiang W, Bergh A, Wester P. Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion. Acta Neuropathol (Berl). 2001 Sep;102(3):216-26. 3. Tille JC, Wang X, Lipson KE, McMahon G, Ferrara N, Zhu Z, Hicklin DJ, Sleeman JP, Eriksson U, Alitalo K, Pepper MS. Vascular endothelial growth factor (VEGF) receptor-2 signaling mediates VEGF-C(deltaNdeltaC)- and VEGF-A-induced angiogenesis in vitro. Exp Cell Res. 2003 May 1;285(2):286-98. 4. Krishnan J, Kirkin V, Steffen A, Hegen M, Weih D, Tomarev S, Wilting J,Sleeman JP. Differential in vivo and in vitro expression of vascular endothelial growth factor (VEGF)-C and VEGF-D in tumors and its relationship to lymphatic metastasis in immunocompetent rats. Cancer Res. 2003 Feb 1;63(3):713-22. 5. Weich HA, Bando H, Brokelmann M, Baumann P, Toi M, Barleon B, Alitalo K, Sipos B, Sleeman J. Quantification of vascular endothelial growth factor-C (VEGF-C) by a novel ELISA. J Immunol Methods. 2004 Feb 15;285(2):145-55.
Tips:Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Additional isotype:IgG
Group:Polyclonals and antibodies
Latin name:Oryctolagus cuniculus,Rattus norvegicus

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