Mouse Monoclonal IgG1, k Anti-bcl-2 Oncoprotein [124]

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Catalog number:BCL002-0.5
Name:Mouse Monoclonal IgG1, k Anti-bcl-2 Oncoprotein [124]
Size:0.5 ml
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Availability:Please contact us to check the availability
Immunogen:Synthetic peptide corresponding to human bcl-2 molecule aa 41-54 (GAAPAPGIFSSQPG-Cys)
Specificity:Human bcl-2 oncoprotein
Species reactivity:Human, not mouse and rat
Product presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1, k
Antibody's clone:124
Product is supplied as:liquid concentrate
Recommended concentration for use:1:100-1:200
Protein concentration:N/A
Positive control:Tonsil
Pretreatment:Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)
Tested applications:IHC(C, P)
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Storage method:Store at 2-8°C
UniProt number:P1Go to UniProt/SwissProt415 (BCL2_HUMAN)
Litterature:1. Tsujimoto Y. and Crone C.M. (1986) Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Nat. Acad. Sci. (USA) 83; 5214-5218. 2. Hockenbery D., Nunez G., Milliman C., et al. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348; 334-336. 3. Liu Y.J., Mason D.Y., Johnson G.D., et al. (1991) Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis. Europ. J. Immunol. 21; 1905-1910. 4. LeBrun D.P., Kamel O.W., Cleary M.L., et al. (1992) Follicular lymphomas of the gastrointestinal tract. Pathologic features in 31 cases and bcl-2 oncogenic protein expression. Am J. Pathol. 140; 1327-1335.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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