Sheep Polyclonal IgG Anti-Complement Factor C3c::FITC

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Catalog number:C3C001-F
Name:Sheep Polyclonal IgG Anti-Complement Factor C3c::FITC
Size:1 ml
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Availability:Please contact us to check the availability
Immunogen:Purified Complement 3 from human serum
Species reactivity:Human
Product presentation:Ion-exchanged purified IgG conjugated with Fluorescein Isothiocyanate Isomer 1 in PBS pH 7.2; contains sodium azide (0,09%)**
Product category:Primary Antibody
Antibody's raised in:Sheep
Antibody's isotype:IgG
Antibody's clone:polyclonal
Product is supplied as:liquid concentrate
Recommended concentration for use:1:50 - 1:100
Protein concentration:10 mg/ml
Positive control:Skin (basal membrane) in bullous pemphigoid
Tested applications:IHC (F); The specified dilution has to be evaluated individually for optimal results.
Storage method:Store at 2-8°C, protected from light
UniProt number:PGo to UniProt/SwissProt1Go to UniProt/SwissProt24-CO3_HUMAN
Litterature:1. Bernard P., Aucouturier P., Denis F., and J.M. Bonnetblanc (1990) Immunoblot analysis of IgG subclasses of circulating antibodies in bullous pemphigoid. Clin. Immunol. Immunopathol. 54(3); 484-494. 2. Feliciani C., Pour S.M., Toto P., Coscione G., and Amerio P. (1998) Direct immunofluorescence diagnosis of pemphigus without biopsy. J. Cutan. Med. Surg. 2(4); 209-211. 3. Buschman K.E., Seraly M., Thong H.Y., Deng J.S., Draviam R.P., and Abernet J.L. (2002) A predominant IgG4 subclass may be responsible for false-negative direct immunofluorescence in bullous pemphigoid. J. Cutan. Pathol. 29(5); 282-286.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Properties:This bio logo Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. bio logo FITC is highly sensitive to pH extremes.
Conjugation:Anti-FITC Antibody
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Additional isotype:IgG
Group:Polyclonals and antibodies

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