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Rabbit Monoclonal IgG Anti-Beta-Catenin [E247]

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Catalog number:CTN001-G
Name:Rabbit Monoclonal IgG Anti-Beta-Catenin [E247]
Size:5 ml
Price:239.00
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Availability:Please contact us to check the availability
Immunogen:Synthetic peptide corresponding to a N-terminal aa sequence of human ß-Catenin
Specificity:Beta-Catenin (CTNB1); Catenin (Cadherin-associated Protein) beta 1
Species reactivity:Human, Mouse, other species not tested
Product presentation:Cell culture supernatant with PBS, glycerol, BSA, and NaN3 (0,09%)
Product category:Primary Antibody
Antibody's raised in:Rabbit
Antibody's isotype:IgG
Antibody's clone:E247
Product is supplied as:ready to use
Recommended concentration for use:RTU
Positive control:Fibromatosis, Colon Carcinoma
Pretreatment:Formalin-fixed materials require heat induced antigen retrieval with citrate buffer (Art. No. DE000) or TEC-Buffer pH 8 (Art. No. DE005). We recommend an unmasking procedure for 30 min at 100°C.
Tested applications:IHC(C, P), WB
Recommended secondary reagent:Anti-rabbit IgG:Biotin conjugate (code no. ZU101) in conjunction with Streptavidin-HRPO (code no. ZU054) or VECTOR ImmPress rabbit IgG Kit (code no. MP-7401).
Storage method:Store at 2-8°C
UniProt number:P35222 (CTNB1_HUMAN)
Litterature:1. Montgomery E, Torbenson MS, Kaushal M, Fisher C, Abraham SC.(2002) Beta-catenin immunohistochemistry separates mesenteric fibromatosis from gastrointestinal stromal tumor and sclerosing mesenteritis. Am J Surg Pathol. 2002 Oct;26(10):1296-301. 2. Montgomery E, Folpe AL. (2005) The diagnostic value of beta-catenin immunohistochemistry. Adv Anat Pathol. 2005 Nov;12(6):350-6. 3. Images of Beta-Catenin in normal mamma tissue and mamma adenoma may be found with the following link:
Tips:** The ß-Catenin antibody is registrated and produced under US Patent No. 5675063 and 7429487. Gentaur Reagents are research reagents for in vitro applications only. They are not intended for clinical diagnostik use. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype:IgG
Latin name:Oryctolagus cuniculus

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