Mouse Monoclonal IgG1 Anti-Alpha-DEFENSIN (1-3), HNP1-3 [DEF-3]

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Catalog number:HNP103
Name:Mouse Monoclonal IgG1 Anti-Alpha-DEFENSIN (1-3), HNP1-3 [DEF-3]
Size:100 µg
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Extra details

Availability:Please contact us to check the availability
Immunogen:Native Defensins
Specificity:Human Neutrophil Peptides (HNP1-3) syn. alpha-Defensins 1-3, reactivity with HNP-4 not tested
Species reactivity:Human, cross-reactivity with rhesus monkey and primates expected
Product presentation:Affinity purified IgG, lyophilized from PBS, BSA, and sodium azide**, reconstitute with 0.5 ml sterile water to get a stock solution of 200 µg/ml.
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1
Antibody's clone:DEF-3
Product is supplied as:lyophilisate
Recommended concentration for use:0.2 µg/ml IHC(C), 0.5 µg/ml IHC(P)
Protein concentration:200 µg/ml IgG after reconstitution with 0,5 ml distilled water
Positive control:Infected skin or oral mucosa
Pretreatment:Formaldehyde-fixed tissue requires antigen unmasking preferred methods are 0.1% Pronase, Proteinase K or Trypsin.
Tested applications:IHC(C, P), FACS
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Storage method:Store at -20'°C'
UniProt number:Go to UniProt/SwissProt
Litterature:1. Ganz T. et al. (1985) J. Clin. Invest., 76; 1427 ff 2. Ganz T., et al. (1992) Med. Microbiol. Immunol. 181; 99ff 3. Mizukawa N ; Sugiyama K ; Fukunaga J ; Ueno T ; Mishima K ; Takagi S ; Sugahara T (1998) Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients. Anticancer Res 18(6B):4645-4649. 4. Linzmeier R., Ho C.H., Hoang B.V. , and Ganz T. (1999) A 450 kb contig of defensin genes on human chromosome 8p23. Gene 233; 205-211. 5. Mikuzawa N. Sugiyama K., Kamio M., Yamachika E., Ueno T., Fukunaga J., Takagi S., Sugahara T. (2000) Immunohistochemical staining of human alpha-defensin-1 (HNP-1) in submandibular glands of patients with oral carcinomas. Anticancer Res. 20(2B); 1125-1127.
Tips:Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:The Mouse Monoclonal IgG1 Anti-Alpha-DEFENSIN (1-3) HNP1-3 [DEF-3] is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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