Mouse Monoclonal IgG2a Anti-GCDFP-15 (gross cystic disease fluid protein-15) [23A3]

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Catalog number:GCD001
Name:Mouse Monoclonal IgG2a Anti-GCDFP-15 (gross cystic disease fluid protein-15) [23A3]
Size:0.5 ml
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Availability:Please contact us to check the availability
Immunogen:Recombinant human GCDFP-15
Specificity:Human GCDFP-15
Species reactivity:Human
Product presentation:Cell culture supernatant PBS buffered, stabilized with BSA and NaN3
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG2a
Antibody's clone:23A3
Product is supplied as:liquid concentrate
Recommended concentration for use:1:50-1:500
Protein concentration:S/N
Positive control:Mamma carcinoma, skin
Pretreatment:For formaldehyde-fixed material we recommend antigen unmasking with citrate buffer (Unmasking Fluid C, Art. No. DE000 or Unmasking Fluid G, Art. No. DE007) at 90-100°C
Tested applications:IHC(C, P), WB
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Storage method:Store at 2-8°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Bhargava R, Beriwal S, Dabbs DJ. (2007) Mammaglobin vs GCDFP-15: an immunohistologic validation survey for sensitivity and specificity. Am J Clin Pathol. 127(1):103-13. 2. Takeda Y., Tsuta K., Shibuki Y., Hoshino T., Tochigi N., Maeshima A.M., Asamura H., Sasajima Y., Ito T., Matsuno Y. (2008) Analysis of Expression Patterns of Breast Cancer–Specific Markers (Mammaglobin and Gross Cystic Disease Fluid Protein 15) in Lung and Pleural Tumors. Archives of Pathology and Laboratory Medicine 132; 239–243. 3. Yan Z., Gidley J., Horton D., Robertson J., Eltourn IE., Chhieng DC. (2009) Diagnostic utility of mammaglobin and GCDFP-15 in the identification of metastatic breast carcinoma in fluid specimens. Diagnostic Cytopathoogy 37(7); 475-478.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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