Mouse Monoclonal IgG1 Anti-NSE, Gamma-Enolase [MID-N3]

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About product

Catalog number:NS100
Name:Mouse Monoclonal IgG1 Anti-NSE, Gamma-Enolase [MID-N3]
Size:1 ml
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Availability:Please contact us to check the availability
Immunogen:Purified human gamma-gamma-enolase
Specificity:Gamma-gamma isoform of enolase
Species reactivity:Human, guinea pig
Product presentation:Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Product category:Primary Antibody
Antibody's raised in:Mouse
Antibody's isotype:IgG1
Antibody's clone:MID-N3
Product is supplied as:liquid concentrate
Recommended concentration for use:1:10-1:50
Protein concentration:50 µg/ml
Positive control:Adrenal medulla, nerve tissue
Pretreatment:No pre-treatment with proteases necessary, improvement of staining with the usage of unmasking fluid G (Art. No. DE007)
Tested applications:IHC(P)
Recommended secondary reagent:We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Storage method:Store at 2-8°C
UniProt number:Go to UniProt/SwissProt
Litterature:1. Marangos P.J., Schmechel D., Parma A.M., Clark R.L., and Goodwin F.K. (1979) Measurement of neuron-specific (NSE) and non-neuronal (NNE) isoenzymes of enolase in rat, monkey and human nervous tissue. J. Neurochem. 33; 319-329. 2. Cras P., Soler Federsppiel S., Gheuens J., Martin J.-J., Löwenthal A. (1986) Demonstration of neuron-specific enolase in nonneuronal tumors using a specific monoclonal antibody. Ann. Neurol. 20; 106-107. 3. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442. 4. Cras P., Martin J.J., and Gheuens J. (1998) Gamma-enolase and glial fibrillary acidic protein in nervous system tumors. An immunohistochemical study using a specific monoclonal antibody. Acta Neuropathol. 75; 377-384.
Tips:*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus

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