Rabbit Polyclonal IgG Anti-VEGF-A, murine

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About product

Catalog number:VEGA03A
Name:Rabbit Polyclonal IgG Anti-VEGF-A, murine
Size:50 µg
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Extra details

Availability:Please contact us to check the availability
Immunogen:Highly purified recombinant murine VEGF164
Specificity:VEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions)
Species reactivity:Human, Mouse
Product presentation:Peptide affinity purified using murine VEGF164 as matrix. Lyophilised from PBS pH 7.4 without preservative.
Product category:Primary Antibody
Antibody's raised in:Rabbit
Antibody's isotype:IgG
Antibody's clone:polyclonal
Product is supplied as:lyophilisate
Recommended concentration for use:Direct ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml
Protein concentration:50 µg
Positive control:Metastasing tumours of different origin
Pretreatment:Formaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.
Tested applications:IHC(C, P), ELISA, WB
Recommended secondary reagent:As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
Storage method:Store at -20°C
UniProt number:QGo to UniProt/SwissProtGo to UniProt/SwissProt731 (VEGFA_MOUSE)
Tips:Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype:IgG
Group:Polyclonals and antibodies
Latin name:Oryctolagus cuniculus

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