Rabbit Anti-Human LC3B Polyclonal: Biotin

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About product

Catalog number:SPC-217A-BI
Name:Rabbit Anti-Human LC3B Polyclonal: Biotin
Size:50 µg
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Extra details

Immunogen:Peptide derived from C-terminal region of human LC3B protein
Antibody's target:Human LC3B
Antibody's full description:Rabbit Anti-Human LC3B Polyclonal
Primary research fields:Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart
Antibody's category:Polyclonal Antibodies
Antibody's other name:MAP1LC3B Antibody, Autophagy related protein LC3 B Antibody, Autophagy related ubiquitin like modifier LC3 B Antibody, Autophagy-related protein LC3 B Antibody, Autophagy-related ubiquitin-like modifier LC3 B Antibody, LC3 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3-like protein 2 Antibody, MAP1A/1B light chain 3 B Antibody, MAP1A/1BLC3 Antibody, Antibody, MAP1A/MAP1B LC3 B Antibody, MAP1A/MAP1B light chain 3 B Antibody, Antibody, MAP1BLC3 Antibody, Antibody, Map1lc3b Antibody, Antibody, Microtubule associated protein 1 light chain 3 beta Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody, Microtubule associated proteins 1A/1B light chain 3A Antibody, Microtubule associated proteins 1A/1B light chain 3B Antibody, Microtubule-associated protein 1 light chain 3 beta Antibody, Microtubule-associated proteins 1A/1B light chain 3B Antibody, MLP3B_HUMAN Antibody
Verified applications:WB
Raised in:Rabbit
Antibody's reactivity:Human, Mouse, Rat
Antibody's recommended dilutions for use:WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody's purified from:Protein A Purified
Recommended buffer for storage:PBS, 15mM sodium azide
Antibody's concentration:See included datasheet or contact our support service
Antibody's specificity:Specific for LC3B. Does not detect LC3A, LC3C, GABARAP, GABARAPL1 or GABARAPL2. Detects LC3B-I and LC3B-II.
Storage recommendations:4°C
Shipping recommendations:Blue Ice or 4°C
Certificate of analysis:A 1:10000 dilution of SPC-217 was sufficient for detection of LC3B in lysate from cells expressing Recombinant GST-tagged LC3B by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-anchor, Cytoplasmic Vesicle, Autophagosome, Autophagosome Membrane
Tissue specificity:See included datasheet or contact our support service.
Scientific context:Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
Bibliography:1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
Released date:12/Jun/2014
NCBI number:NP_073729.12
Gene number:81642
Protein number:Q9GZQ19
PubMed number:Refer to PubMed
Tested applications:To be tested
Tested reactivity:To be tested
Antibody's datasheet:Contact our support service to receive datasheet or other technical documentation.
Representative figure link:No Data Available
Warning information:Non-hazardous
Country of production:Canada
Total weight (kg):1.4
Net weight (g):0.05
Stock availabilit:In Stock
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test:StressMark antibodies supplies antibodies that are for research of human proteins.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group:Polyclonals and antibodies
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name:Oryctolagus cuniculus

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