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Rabbit Anti-Mouse GRP94 Polyclonal: Biotin

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About product

Catalog number:SPC-101D-BI
Name:Rabbit Anti-Mouse GRP94 Polyclonal: Biotin
Size:100 µg
Price:362.00
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Extra details

Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
Antibody's target:Mouse GRP94
Antibody's full description:Rabbit Anti-Mouse GRP94 Polyclonal
Primary research fields:Cancer, Heat Shock, Cell Signaling, Trafficking
Antibody's category:Polyclonal Antibodies
Antibody's other name:HSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody
Verified applications:WB, IHC, ICC/IF, IP
Raised in:Rabbit
Antibody's reactivity:Human, Mouse, Rat, Bovine
Antibody's recommended dilutions for use:WB (1:1000), ICC/IF (1:120), IP (1:80); optimal dilutions for assays should be determined by the user.
Antibody's purified from:Peptide Affinity Purified
Recommended buffer for storage:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration:1 mg/ml
Antibody's specificity:Detects ~94kDa.
Storage recommendations:-20°C
Shipping recommendations:Blue Ice or 4°C
Certificate of analysis:1 µg/ml of SPC-101 was sufficient for detection of Grp94 in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell:Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome
Tissue specificity:See included datasheet or contact our support service.
Scientific context:Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
Bibliography:1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128. 2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98. 3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883. 4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649. 5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338. 6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269. 7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637. 8. Allen S. et al. (2000) Blood 96(2): 560-568. 9. Sato K et al. (2001) Blood 98(6): 1852-1857. 10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378. 11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858. 12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386. 13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104. 14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
Released date:25/Jul/2012
NCBI number:NP_035761.1
Gene number:22027
Protein number:P08113
PubMed number:Refer to PubMed
Tested applications:To be tested
Tested reactivity:To be tested
Antibody's datasheet:Contact our support service to receive datasheet or other technical documentation.
Representative figure link: | |
Representative figure legend:Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Rat brain cell lysates showing detection of ~ 94-100 kDa GRP94 protein using Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101). Lane 1: MW ladder. Lane 2: Anti-GRP94 (1:250). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101) at 1:250 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~ 94-100 kDa. Other Band(s): ~50, ~75 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody (SPC-101) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-GRP94 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-101) | Rabbit Anti-GRP94 Antibody used in Western blot (WB) on Rat brain cell lysates (SPC-101) | Rabbit Anti-GRP94 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-101)
Warning information:Non-hazardous
Country of production:Canada
Total weight (kg):1.4
Net weight (g):0.1
Stock availabilit:In Stock
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation:Biotinylated
Test:Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name:Mus musculus,Oryctolagus cuniculus
Group:Polyclonals and antibodies
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

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