Bioxys

Rabbit anti Cat IgG (heavy and light chains) antibody conjugated with Biotin

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About product

Catalog number:RACa/IgG(H+L)/Bio
Name:Rabbit anti Cat IgG (heavy and light chains) antibody conjugated with Biotin
Size:1 mL
Price:224.00
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Extra details

Category:Secondary Antibodies
Long description:The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in cat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of cat origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:20,000.
Antibody come from:Purified normal IgG isolated from pooled cat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Other description:Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
Clone:Polyclonal
Antigen-antibody binding interaction:Rabbit anti Cat IgG (heavy and light chains) antibody conjugated with Biotin Antibody
Antibody is raised in:Rabbit
Antibody's reacts with:Feline
Antibody's reacts with these species:Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine - Chicken - Dog + Duck - ferret + Goat -Guinea Pig ± Hamster ± Horse - Human + Mouse - Monkey ± pigeon - Rat + Sheep ± Swine ± Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
Antibody's specificity:Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Cat IgG, heavy and light chains.
Research interest:Veterinary
Application:Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
Antibody's suited for:Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
Storage:The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
Relevant references:no information yet
Protein number:see ncbi
Warnings:This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Felix vulgaris genes or proteins used as antigen, synthetic peptide.
Conjugation:Biotinylated
About:Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Additional isotype:IgG
Latin name:Oryctolagus cuniculus
French translation:anticorps

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